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Urine-Based Assay Diagnoses Chlamydia in 20 Minutes

A rapid and sensitive assay requiring no special equipment can detect Chlamydia trachomatis directly from urine samples with high accuracy, according to a study published in the January issue of the Journal of Molecular Diagnostics.

Katrin Kr├Álov, from the University of Tartu in Estonia, and colleagues describe an assay to detect C. trachomatis in urine samples using recombinase polymerase amplification of a highly conserved target within the CDS2 gene. The assay was tested on urine samples from 70 patients attending a sexual health clinic and compared with the Roche Cobas Amplicor C. trachomatis assay.

The researchers found that the test had a minimum detection limit of five to 12 pathogens and could detect pathogen directly in urine within 20 minutes. Initial analysis of the assay showed specificity of 100 percent and a sensitivity of 83 percent.

"The whole procedure is fairly simple and does not require specific machinery, making it potentially applicable in point-of-care settings," Krolov and colleagues conclude.

The abstract and full text may be accessed here.

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