“Until we have a valid drug treatment strategy or vaccine against COVID-19, we will have to perform mass blood testing in our corresponding countries,” says Igor Stagljar, a professor of biochemistry and molecular genetics in the Faculty of Medicine and the Donnelly Centre for Cellular and Biomolecular Research who leads the effort with support from the Toronto COVID-19 Action Fund.
“These serological tests will be the only way we can reliably determine the extent of viral spread and identify those individuals who are able to safely return to work.”
Serological tests look for antibodies in blood that are produced by the body to fight infection. Antibodies are protein molecules that bind and neutralize foreign proteins, known as antigens, that come from viruses and other pathogens.
According to the plan outlined by the federal COVID-19 Immunity Task Force, at least one million serological tests will be carried out over the next two years to help gauge how many Canadians have been exposed to the novel coronavirus.
Health Canada recently announced it approved the first serological test for COVID-19. However, concerns have been raised over the ability of some methods to detect potentially low antibody levels in people who were recently infected or suffered mild or no symptoms.
“Ideally, we want to have a test that is very fast, low cost and accurate,” says Stagljar, whose team is working to achieve just that.
The U of T team’s test, which works in under an hour, involves dousing a blood sample with purified SARS-CoV-2 proteins so that antibodies can bind to them. The viral proteins are engineered to carry pieces of a fluorescent protein that can only be reconstituted if antibodies are present in a chemical reaction that produces a flash of light – a positive result – that is detected by a plate reader instrument.
The test also employs a new technology called SIMPL, which stands for split intein mediated protein ligation, developed by Stagljar’s team and described in a study published recently in the journal Nature Communications. SIMPL helps strengthen the contact between antibodies and viral proteins to increase test sensitivity.
“We originally devised SIMPL to study interactions between proteins in living cells, however, we quickly realized that the technology could be adapted to dramatically improve the sensitivity of a COVID-19 serological assay” says Zhong Yao, a senior research associate in Stagljar’s lab who is responsible for both the COVID-19 and SIMPL projects.
The prototype lab test can detect different types of antibodies such as immunoglobulinM and immunoglobulinG that can help distinguish how long ago an infection occurred. IgM antibodies are produced first and are relatively short-lived, while IgG antibodies come later and linger in the bloodstream to confer longer-lasting immunity. While is still unclear how long the immunity to COVID-19 lasts, serological tests will help answer this.
“Our goal is to detect IgM and IgG antibodies in only 10 microliters of blood,” says Stagljar.
Having validated their test with purified antibodies, the team are now calibrating it for the detection of variable antibody levels in patient blood samples. “We should know in four months if it’s working or not.”
Stagljar is collaborating with Samira Mubareka, an assistant professor in the department of laboratory medicine and pathobiology in the Faculty of Medicine who is at Sunnybrook Health Sciences Centre, and Mario Ostrowski, a professor in the departments of medicine, immunology and laboratory medicine and pathobiology who is at St. Michael’s Hospital. James Rini, a professor of molecular genetics and biochemistry in U of T’s Faculty of Medicine, is also involved in the research.
The new SIMPL method locks interacting proteins together to help capture transient protein interactions in cells that could not be detected with other methods. It can also be applied across different types of human cells and to study interactions that occur in different cellular compartments.
Aside from the antibody test, Stagljar is applying SIMPL for cancer drug discovery. One project is looking for small molecules that can inhibit protein interactions involving disease variants of the KRAS protein, which is mutated in a third of all cancers. The project has received funding from the Cancer Research Society, FACIT Oncology Fund, Genome Canada and Toronto Innovation Acceleration Partners, a public-private partnership supporting academic research commercialization and previously known as Mars Innovation.
Source: University of Toronto News