Traditional biological methods for flu virus detection include genetic analysis -- reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) used in immunology. However, RT-PCR is expensive and time-consuming while the sensitivity for ELISA is relatively low. Such limitations make them difficult for clinical use as a front-line and on-site diagnostic tool for virus detection, paving the way for PolyU's development of the new upconversion nanoparticle biosensor which utilizes luminescent technique in virus detection.
PolyU's researchers have developed a biosensor based on luminescent technique which operates like two matching pieces of magnet with attraction force. It involves the development of upconversion nanoparticles (UCNPs) conjugated with a probe oligo whose DNA base pairs are complementary with that of the gold nanoparticles (AuNPs) flu virus oligo. Given the complementary nature of the DNA base pairs of the UCNPs probe oligo and AuNPs flu virus oligo, they work like two matching pieces of magnet, which would be drawn together due to attraction force. This process is also called oligo hybridization. Upon being illuminated by a portable near-infrared laser pen, the UCNPs emit eye-visible green light while the AuNPs would absorb the green light. One can easily quantify the concentration of the targeted flu virus by measuring the decrease in the green light intensity.
Initially, PolyU researchers have utilized upconversion LRET for ultrasensitive virus detection in liquid phase system. The research team has further improved the sensitivity of the luminescent detection method by utilizing a solid phased nanoporous membrane system (NAAO) for virus detection. As NAAO membrane consists of many hollow channels, they allow more space for oligo hybridization to take place, significantly increasing its sensitivity by more than 10 folds compared to the liquid phase system, proven by clinical detection using inactivated virus samples.
Not only is the design and operation of PolyU's invention simple, it does not require expensive instruments and sophisticated operational skills, with its sensitivity comparable to traditional clinical methods. In comparison to conventional downconversion luminescent technique, it causes low damage to genetic materials and does not induce background fluorescence. In addition, since each virus has a unique genetic sequence, researchers would be able to design a complementary probe once the genetic sequence of the targeted virus is known. In other words, the upconversion LRET technology can be widely used for the detection of different types of viruses simply by modifying the UCNPs capture probe.
The related results have been recently published in ACS Nano and Small, two leading journals in nano material research. With the support from the Innovation and Technology Support Programme, the research team will continue to enhance the nano biosensor for rapid virus detection, which includes increasing its sensitivity and specificity, and developing a matrix for detection of multiple flu viruses on a single testing platform.